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KMID : 0903619980390030316
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Journal of the Korean Society for Horticultural Science
1998 Volume.39 No. 3 p.316 ~ p.322
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Linkage Mapping of Interspecific Population between Malus domestica cv. Fuji and M. baccata Based on RAPD Markers
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예병우/Yae, Byeong Woo
황정환/송관정/서은정/류맹군/신용억/Hwang, Jeong Hwan/Song, Kwan Jeong/Suh, Eun Jung/Liu, Meng Jun/Shin, Youn Uk
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Abstract
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This study was carried out to construct genetic linkage maps for apples based on an interspecific F₁ population of 60 progenies originated from a cross between M. domestica cv. Fuji and M. baccata. Out of 148 Operon 10-mer primers screened, 59 primers showing more than two polymorphic bands between two parents were selected for practical RAPD analysis. A total of 172 RAPD markers were useful for mapping. Markers segreating as a result of heterozygosity only in Fuji, in M. baccata, and in both were 73, 77, and 22, respectively. Morphological traits were checked in the parents and their 60 progenies. Only the form of stipule, entire in Fuji and serrated in M. baccata, showed no significant difference in segregation ratio from 1:1, the Mendelian segregation pattern. From the pseudotestcross and the double pseudotestcross design, initial linkage maps for the two important oriental apple species were constructed based mainly on the RAPD markers. The linkage map for Fuji consists of 19 markers arranged in 5 linkage groups/pairs and extends 245.1 cM. The M. baccata map contains 20 markers on 4 linkage groups and spans 104.0 cM. The most saturated linkage group is group one of M. baccata linkage map which comprises 14 markers with an average distance of 5.96 cM between adjacent markers. The locus for entire stipule was mapped to the longest linkage group of Fuji between two RAPD markers, G18-870 and F07-1780, with a distance of 15.5 and 17.4 cM, respectively, while that for serrated stipule was mapped to the longest linkage group of M. baccata with a distance of 11.6 cM to the RAPD marker E06-1120. The longest linkage groups of Fuji and that of M. baccata were assumed to be homologous.
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